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SDBS对大鳞副泥鳅胚胎发育及幼鱼性腺发育的影响
Effects of SDBS on embryonic development and gonad development of Paramisgurnus dabryanus
【摘要】 为了解十二烷基苯磺酸钠(SDBS)对大鳞副泥鳅(Paramisgurnus dabryanus)胚胎发育及幼鱼性腺发育的影响,将受精卵随机分成7组,分别置于0、0.1、0.3、0.5、1.0、2.5、5.0 mg/L SDBS溶液中充氧孵化,统计培育周期、孵化周期、孵化率和畸形率。选取均重为3 g左右健康的大鳞副泥鳅210尾,随机分成7组,分别置于0、0.1、0.3、0.5、1.0、2.5、5.0 mg/L SDBS溶液中饲养60 d,统计大鳞副泥鳅的死亡情况,取性腺制作组织切片,观察SDBS对大鳞副泥鳅幼鱼性腺发育的影响。结果显示:SDBS浓度≤1.0 mg/L时,对大鳞副泥鳅受精卵培育周期、孵化周期、孵化率和鳅苗畸形率无显著影响,SDBS浓度≥2.5 mg/L时,培育周期与孵化周期显著延长,孵化率显著下降,鳅苗畸形率显著上升。大鳞副泥鳅的死亡率随SDBS浓度的升高明显上升,SDBS浓度≥0.1 mg/L时显著抑制精巢发育。
【Abstract】 In order to investigate the effect of Sodium Dodecyl Denzene Sulfonate(SDBS) on the embryonic development of Paramisgurnus dabryanus,the fertilized eggs of P. dabryanus were randomly divided into 7 groups,3 replicates per group,and each repeating 1 000 grains. Aerobic incubations were performed in SDBS solution at concentrations of 0,0. 1,0. 3,0. 5,1. 0,2. 5,5. 0 mg/L,respectively. Then the incubation cycle,hatching cycle,hatching rate and deformity rate were counted. 210 healthy P. dabryanus with an average of about 3 g were randomly divided into 7 groups,each with 3 replicates,and each repeating 10 tails,were reared in SDBS solution at concentrations of 0,0. 1,0. 3,0. 5,1. 0,2. 5,5. 0 mg/L for 60 days,respectively. During the breeding period,the death of P. dabryanus was recorded. In addition,the effect of SDBS on the gonad development of P. dabryanus treated with 60 d by tissue section was observed. The results showed that the SDBS concentration was less than or equal 1. 0 mg/L,which had no significant effect on the incubation period,hatching cycle,hatching rate and lozar deformity rate. While the SDBS concentration was greater than or equal to 2. 5 mg/L,the incubation cycle and the incubation cycle were significantly prolonged,the hatching rate was markedly decreased and the deformity rate was significantly increased. Besides,the mortality of P. dabryanus was significantly increased with the increase of SDBS concentration,and SDBS concentration was greater than or equal to 0. 1 mg/L significantly inhibited the development of P. dabryanus testis.
【Key words】 sodium dodecyl denzene sulfonate; Paramisgurnus dabryanus; embryonic development; gonad development;
- 【文献出处】 淡水渔业 ,Freshwater Fisheries , 编辑部邮箱 ,2018年01期
- 【分类号】S917.4
- 【被引频次】5
- 【下载频次】196