【摘要】 以当年采收以及贮藏1年和2年的芍药种子为试验材料,对不同贮藏年份的芍药种子进行低温(-20℃、-40℃)及沙藏处理,同时测定处于不同生根阶段的芍药种子的生理生化指标,以明确芍药种子快速生根的途径,探讨芍药种子萌发生根的内在生理机制。结果表明:(1)单因素方差分析显示,贮藏年份、处理温度以及处理天数均对芍药首粒种子生根时间、生根指数、生根率有极显著的主效应,三因素单独对各生根指标都有极显著的影响,其中贮藏年份对种子生根的影响力最大。(2)在同一贮藏年份下,沙藏生根前进行-40℃低温处理30d对芍药种子生根效果最好,生根率最高(88.33%);不同贮藏年份的芍药种子在沙藏生根前进行同一低温处理后,贮藏1年的芍药种子生根效果优于当年采收和贮藏2年的芍药种子。(3)不同贮藏年份的芍药种子在生根过程中生理生化指标变化趋势相似,表现为含水量持续升高,可溶性糖含量先增加后减少,淀粉含量持续减少,可溶性蛋白含量先减少后增加;过氧化物酶活性先升高后降低,过氧化氢酶活性先降低后持续升高。研究发现,-40℃低温处理30d能够有效打破芍药种子下胚轴休眠,促进芍药种子生根,且采收后贮藏1年的芍药种子生根效果最好。更多还原

【Abstract】 Peony(Paeonia lactiflora Pall.)seeds stored for one or two years and newly harvested seeds were used as experiment materials.The seeds were treated with low temperature(-20℃ or-40℃)and then stored in the sand for elucidating the methods for promoting rooting process.Physiological and biochemical indexes were analyzed during different rooting stages for investigating the internal mechanisms.The results showed:(1)One-way analysis of variance showed storage years,treatment temperature and treatment time length performed obviously significant main effects together or independently on rooting index,rooting rate and first-seed rooting time of peony seeds,and storage years performed strongest influence.(2)For peony seeds stored in the same year,treatment of-40℃treatment for 30 days before sand storage performed the best rooting efficiency with the highest rooting rates of 88.33%.When under thesame treatments,the rooting efficiency of one-year-storage peony seeds is better than those of other storage time length.(3)Physiological and biochemical analysis showed that during the seed rooting process,for the peony seeds stored for the same time length,the water content kept raising,the soluble sugar content increased first and then decreased,the starch content kept decreasing,the soluble protein content decreased first and then increased;peroxidase(POD)activity increased first and then decreased,catalase(CAT)activity decreased after an increase.Conclusion:the treatment of-40℃for 30 days can effectively break the dormancy of Paeonia lactiflora Pall.seeds and promote the rooting process,and one-yearstorage seeds performed the best rooting efficiency.更多还原