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大鼠无创伤气管插管心肌缺血再灌注损伤实验的探索与实践
Exploration and practice of experimental study on myocardial ischemia reperfusion injury in rats without traumatic tracheal intubation
【摘要】 在急性心肌缺血再灌注实验中,自制大鼠无创伤气管插管,避免颈部手术和有创伤气管插管对大鼠的损伤,简化手术步骤,降低手术难度,提高实验成功率和动物存活率。改进染色心肌拍照方法,提高照片清晰度和准确度;应用BI-2000图像分析系统测量心肌梗死面积,保证实验结果准确性和可靠性。通过改进和完善实验操作方法,为相关实验教学和科学研究的顺利开展奠定基础。
【Abstract】 In acute myocardial ischemia-reperfusion experiments, homemade rats non-invasive intubation can avoid neck surgery and injury of trauma intubation of for rats, simplify thesurgical procedures, reduce the diffi culty of operation, and improve the success rate of the model and animal survival. Change the photographic method of myocardial staining to improve photo clarity and acuuracy. Use BI-2000 image analysis system measures the myocardial infarct size, to ensure the accuracy and reliability of the experimental results. Through improving and perfecting the experimental operation method, lay the foundation for the successful development of relevant experimental teaching and scientific research.
- 【文献出处】 长春中医药大学学报 ,Journal of Changchun University of Chinese Medicine , 编辑部邮箱 ,2018年05期
- 【分类号】R-33
- 【被引频次】1
- 【下载频次】201