【摘要】 以蛇皮为原料,以胶原蛋白提取率为指标,采用高温酸法提取胶原蛋白。研究了有机酸种类及柠檬酸浓度、提取温度、浸提时间和固液比5个因素对蛇皮胶原蛋白提取率的影响。以单因素试验为基础,通过正交试验优化了蛇皮胶原蛋白的提取工艺。确定酸法提取蛇皮中胶原蛋白的较佳工艺为:以1 mol/L的柠檬酸作为浸提剂,采用1∶60(g∶m L)的固液比,在110℃下提取3 h,此时提取率较高,为87.01%。更多还原

【Abstract】 Taking snake skin as raw material,and the extraction rate of collagen as the index,the high temperature acid method was used to extract collagen. The influences of the organic acid types and the concentration of citric acid,extraction temperature,extraction time and solid-to-liquid ratio on the extraction rate of collagen were studied. Based on single factor experiment,the extraction process of collagen of snake skin was optimized by orthogonal test. The optimized extraction process was obtained by acid extraction method which is found at the conditions of 1 mol/L initial concentration of citric acid as extraction agent with the solid-to-liquid ratio of 1∶ 60( g∶ m L),at a temperature of 110 ℃ of 3 h,the extraction rate is 87. 01%.更多还原