【摘要】 探讨不同配比18α-与18β-甘草酸(Gly)对大鼠酒精性肝损伤的影响.健康SD大鼠,雄性,随机分组,每组10只,即正常对照组(生理盐水)、模型对照组(生理盐水)、阳性药物对照组(水飞蓟宾)、7个给药组(18α-Gly与18β-Gly不同配比组,配比为10∶0,8∶2,6∶4,5∶5,4∶6,2∶8,0∶10).给药4周后,检测不同组对血清酶、血清蛋白、总胆红素、胆汁酸及血糖调控的影响.结果显示:18α-Gly与18β-Gly各个配比均能降低血清酶ALT、AST、γ-GT、LDH活性,随着18β-Gly比例增加作用增强,当18α-Gly与18β-Gly配比小于4∶6时,对缓解酒精性肝损伤和心肌损伤效果更佳;当配比大于8∶2时,对酒精性肝损伤大鼠血清蛋白水平的改善作用更加显著.当配比小于4∶6时,明显降低TBIL和TBA水平,显著改善酒精性肝损伤大鼠对血糖的调控能力.研究结果为新药研发和临床治疗的制剂选择提供依据.更多还原

【Abstract】 The objective is that investigating protective effects of different proportion of 18α-and 18β-glycyrrhizic acid( Gly) on alcoholic liver injury in rats. Healthy male rats are randomly divided into equally ten groups,respectively normal control group( saline),model group( saline),positive drug control group( silymarin),7 given drug groups( different ratios of 18α-Gly and 18β-Gly group,and ratios are 10∶ 0,8∶ 2,6∶ 4,5∶ 5,4∶ 6,2∶ 8,0∶ 10). At the fourth week,blood are collected to determine serum ferment and proteins,total bilirubin and acid bile. Blood glucose is measured. The results show that different proportions of 18α-and 18β-glycyrrhizic acid all effectively reduced ALT,AST,γ-GT and LDH activity. The alcoholic liver injury and myocardial injury can be better improved when the ratio is less than 4∶ 6. And the serum protein level in alcoholis liver injury rat is improved when the ratio is greater then 8∶ 2. The level of TBIL and TBA. And is attenuated when the ratio is less than 4∶ 6. And the proportion is less then 4∶ 6 that obviously improves the ability of regulating the blood glucose of rats with alcoholic liver injury. It can be applied to selection of new drug research and clinical treatment with a basic experiment data.更多还原