【摘要】 目的:建立金感胶囊中盐酸金刚烷胺的含量测定方法。方法:采用气相色谱法,色谱柱为ZB-5毛细管柱(30m×0.32mm,0.25μm),FID检测器,进样口温度为250℃,检测器温度为300℃,柱温为程序升温,初始柱温为100℃,保持2min,以20℃/min的速率升温至220℃,再以60℃/min的速率升温至280℃,保持1min,载气为氮气,恒压模式,柱前压为70kPa,分流进样,分流比5∶1。结果:盐酸金刚烷胺进样浓度在0.05016-1.00320mg/mL线性关系良好(r=0.9998),平均回收率(n=9)为100.9%。阴性样品不干扰测定。结论:所建立的毛细管气相色谱法简单、灵敏、准确、重复性好,可用于金感胶囊中盐酸金刚烷胺含量的测定及质量控制。更多还原

【Abstract】 Objective: To establish a gas chromatography(GC) method for the determining the content of amantadine hydrochloride in Jingan Capsule. Methods: The GC was performed on a ZB-5 capillary column(30 m×0.32 mm, 0.25μm) with a flame ionization detector(FID). The injector temperature was 250oC and detector temperature was set at 300oC. The temperature program of column oven was set as follows: 100oC for 2 min, increased to 220oC at a rate of 20oC/min, then at 60oC/min to 280oC, held for 1 min. The carrying gas was nitrogen(70 k Pa), and the injection spilt ratio was 5:1. Results: The calibration curve of amantadine hydrochloride was in good linear correlation in the concentration rang of 0.05016-1.00320 mg/mL(r=0.9998). The mean recovery(n=9) was 100.9%. Negative sample did not interfere with the determination. Conclusion: This established method is simple, sensitive, accurate and reproducible. It can be used for determination of amantadine hydrochloride in Jingan Capsule and the quality control of Jingan Capsule.更多还原