【摘要】 目的:研究多囊蛋白1(PC1)对人宫颈癌HeLa细胞凋亡的影响,为进一步阐明PC1对凋亡的作用提供依据。方法:采用PC1基因重组质粒pEGPF-PC1-5TMC和空白对照质粒pEGFP分别转染HeLa细胞,Western blotting法进行验证。采用MTT法分别检测转染组(转染PC1重组质粒)、空白对照组(转染空白对照质粒pEGFP)和正常对照组(正常HeLa细胞)转染后48和72h时细胞增殖活性,采用TUNEL法检测转染组和空白对照组细胞凋亡率。结果:转染后48和72h,与正常对照组比较,转染组细胞增殖活性差异无统计学意义(P>0.05);与空白对照组比较,转染组细胞增殖活性明显升高(P<0.05)。转染组细胞凋亡率明显低于空白对照组(P<0.01)。结论:PC1基因对人宫颈癌HeLa细胞有抗凋亡作用。更多还原

【Abstract】 Objective:To investigate the effect of polycystin-1(PC1)on the apoptosis of human cervical cancer HeLa cells,and to provide the basis for further clarifying the effect of PC1 on apoptosis.Methods:The recombinant plasmid pEGPF-PC1-5 TMC of PC1 gene and blank control plasmid pEGFP were respectively used to transfect the HeLa cells,which was verified by Western blotting method.The proliferation activities of cells in transfection group(transfected with recombinant PC1 plasmid),blank control group(transfected with blank control plasmid pEGFP)and normal control group(normal HeLa cells)were respectively detected by MTT at48 and 72 hafter transfection.The apoptotic rates of the cells in transfection group and blank control group were detected by TUNEL method.Results:Compared with normal control group,the proliferation ability of HeLa cells in transfection group had no significant difference at 48 and 72 hafter transfection(P>0.05);compared with blank control group,the proliferation ability of HeLa cells in transfection group was increased significantly(P<0.05)at 48 and 72 hafter transfection.The apoptotic rate of HeLa cells in transfection group was obviously lower than that in blank control group(P<0.01).Conclusion:PC1 gene has anti-apoptosis effect on the human cervical cancer HeLa cells.更多还原