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不同浓度10%小檗碱对番茄幼苗的影响

Effect of Different Concentrations of 10% Berberine on Tomato Seedlings

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【作者】 师建华; 齐连芬; 王丹丹; 李燕; 葛喜珍; 田国英;

【Author】 SHI Jianhua;QI Lianfen;WANG DANDan;LI Yan;GE Xizhen;TIAN Guoying;Shijiazhuang Academy of Agricultural and Forestry Sciences;Biochemical Engineering College,Beijing Union University;

【通讯作者】 田国英;

【机构】 石家庄市农林科学研究院; 北京联合大学生物化学工程学院;

【摘要】 以"阿里山"番茄为试验材料,采用穴盘育苗方式,设置0(CK)、8、16、32、64、128mg·L-1不同浓度10%小檗碱,分别测定番茄出苗率、生理指标、相关酶活性,确定番茄幼苗生长的最适宜10%小檗碱(BBR)可湿性粉剂浓度。结果表明:32 mg·L-1和64mg·L-1处理的番茄幼苗出苗最快,出苗率最高;32mg·L-1处理下番茄叶绿素含量和过氧化物酶活性均最高,分别为0.76mg·g-1、4 802.89U·g-1·min-1 FW,比CK分别提高16.43%、47.45%。32mg·L-1处理的番茄幼苗丙酮酸激酶和α-淀粉酶活性均最低,分别为505.18U·min-1,6.22mg·g-1·min-1,比CK分别降低10.77%、7.95%。表明番茄幼苗生长最佳的10%小檗碱浓度为32mg·L-1。

【Abstract】 Tomato variety of ‘Alishan’ was used as the experimental material,six different concentrations of 10% berberine(0,8,16,32,64,128 mg·L-1)were set,to study the effect of different concentrations of 10% berberine(BBR)WP on germination rate and the physiological index of tomato enzyme activity of tomato seedlings by plug seedling raising method,and to determine the optimum concentration of 10% berberine for the growth of tomato seedlings.The results showed that,tomato seedlings treated with 32 mg·L-1 and 64 mg·L-1 had the highest emergence rate and the highest emergence rate.32 mg·L-1 10% berberine treatment of tomato seedlings had the highest chlorophyll content and peroxidase activity,which were 0.76 mg·g-1 and 4 802.89 U·g-1·min-1 FW,increased 16.43%,47.45%,respectively.The pyruvate kinase andα-amylase activities of tomato seedlings treated with32 mg·L-1 were the lowest,which were 505.18 U·min-1 and 6.22 mg·g-1·min-1,respectively,and decreased 10.77% and 7.95% compared to the control treatment,respectively.It showed that the best 10% berberine concentration in tomato seedlings was 32 mg·L-1.

【关键词】 番茄幼苗; 10%小檗碱; 叶绿素; 酶活性;
【Key words】 tomato seedlings; 10% berberine; chlorophyll; enzyme activity;
【基金】 河北省科技计划资助项目(17226915D);石家庄市科学技术研究与发展计划资助项目(161490132A)
  • 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2018年16期
  • 【分类号】S641.2
  • 【下载频次】93
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