【摘要】 以多肉植物玉扇为试材,研究了外植体选择、培养基激素配比对玉扇组织培快繁的影响,以期优化玉扇的组织培养条件,建立适宜于工厂化育苗的玉扇组培快繁技术体系。结果表明:以玉扇的根颈横切片作为外植体效果最佳,玉扇最佳诱导愈伤组织培养基为MS+1.0 mg·L-16-BA+0.1 mg·L-1NAA;最佳分生茎叶培养基为MS+2.0 mg·L-16-BA+0.1 mg·L-1NAA;最佳生根培养基为MS+0.1 mg·L-1NAA。更多还原

【Abstract】 Haworthia truncata was taken as test material,effects of selection of explants and proportion of hormone in culture medium of tissue culture of Haworthia truncata were studied. In order to optimize the tissue culture conditions of Haworthia truncata,this test established a tissue culture and rapid propagation system that was suitable for industrial seedling rearing. The results showed that transverse section of root neck of Haworthia truncata was the best as the explant in callus induction stage. The best media for each stages were as follows: the best medium for callus induction was MS + 1. 0 mg·L-16-BA+ 0. 1 mg·L-1 NAA,the best medium for inducing stem and leaf was MS + 2. 0 mg·L-16-BA + 0. 1 mg·L-1 NAA,the best medium for inducting root was MS + 0. 1 mg·L-1 NAA.更多还原