[Objective] The research aimed to detect wheat deoxyger minic acid( DMA) by ultra-high performance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS) serial optimization method.[Method]Five wheat varieties from Shandong Province were selected to study the DMA of root eluent in wheat seedling stage by UPLC/ESI-MS.[Result]The first class mass spectrometry ion peak was m/z 305.2 and the second class mass spectrometry was quantified by positive ions m/z 286.8 and m/z 133.9. The standard product was ide...