[目的]筛选野生红菇娘(Calyx seu Fructus Physalis)和紫菇娘(Physalis tungkenensis Kuan et Gao)组织培养的最佳培养基。[方法]分别以2种植物的茎、叶为外植体,比较不同取样方式、不同增殖条件及不同生根条件对2种植物组织培养和快速繁殖的影响。[结果]以MS+6-BA 3.0 mg/L+NAA 0.04 mg/L为最适合红菇娘愈伤组织诱导和分化的培养基,而紫菇娘是MS+6-BA 3.0 mg/L和NAA0.03 mg/L,培养14 d后每个愈伤组织能产生3~5个不定芽。继代和生根培养后,丛生芽的成活率为90%以上。[结论]该研究可为2种酸浆属野生种质资源保存和优质种苗生产提供技术参考。
【英文摘要】
[Objective]The best tissue culture medium for Calyx seu Fructus Physalis and Physalis tungkenensis Kuan et Gao was selected.[Method]Stem and leaf were used as the explants for the tissue culture and rapid propagation by comparing different sampling methods,different breeding conditions and different rooting conditions.[Result]MS + 6-BA 3. 0 mg/L + NAA 0. 04 mg/L was the best medium for callus induction and bud differentiation of Calyx seu Fructus Physalis,MS + 6-BA3. 0 mg/L + NAA 0. 03 mg/L was the best med...
【基金】
佳木斯大学校级科研课题(S2014-029)
【更新日期】
2018-03-20
【分类号】
S567.239
【正文快照】
红菇娘(Calyx seu Fructus Physalis)为茄科酸浆属多年生宿根草本植物,原产于我国东北,果实味甘,微酸,可以食用;全株可入药,是一种重要的中草药;野生资源匮乏,人工栽培效率不高[1],主要通过传统方式繁殖,如种子繁殖、根茎繁殖等,然而种子发芽需要1~2年,用吸芽或根茎繁殖也较慢,