【摘要】 应用低浓度美兰染色液(2.0%)对原生动物进行梯度微麻醉,麻醉后的虫体活动性减弱但伸缩泡活动状态良好,能够较清晰的观察伸缩泡及收集管的交替活动,可计数活动频率,且这种状态的维持时间较长(约19min)。为将细胞压制为既较薄又不破碎,实验应用低浓度(1%)甲基纤维素作为支撑物,结果表明,该方法操作简单,效果较好,适用于高校或中学动物学实验的课堂操作。更多还原

【Abstract】 Protozoa was carried out with low concentration of methylene blue staining solution(2.0%) to be gradient micro-anesthetized.The activity of the insects after anesthesia was weakened but the activity of the contractile vacuole was good.It was possible to observe the alternating activities of the contractile vacuole and the collecting tube more clearly.The activity frequency can be counted.This state is maintained for a longer period of time(about 19 minutes).To suppress the cells to be both thin and not broken,the experiment used low concentrations(1%) of methyl cellulose as a support.This method has a simple operation and is suitable for classroom operation in laboratory experiments in middle schools or colleges.更多还原