【摘要】 本试验用田间接虫和室内生测方法,研究国产转基因玉米杂交种瑞丰1号-双抗12-5(RF1-12-5)对3种主要的玉米鳞翅目害虫亚洲玉米螟、黏虫、棉铃虫的抗性。田间结果表明:RF1-12-5对亚洲玉米螟、棉铃虫的抗性在相应的鉴定时期均达到高抗水平,RF1-12-5心叶期对黏虫的抗性为抗性水平。室内鉴定结果表明:心叶期,RF1-12-5对亚洲玉米螟抗性起效较慢,饲喂48~72 h后校正死亡率分别为63.97%、77.14%、88.00%;吐丝期饲喂48 h后校正死亡率达100%,抗性显著;籽粒期饲喂24、48、72 h后校正死亡率分别为68.86%、83.96%、98.03%。心叶期饲喂24 h后对黏虫基本无抗性效果,但48、72 h后的校正死亡率分别达到94.64%、100%。吐丝期饲喂24 h后对棉铃虫抗性达到87.59%,48 h后对棉铃虫校正死亡率达到100%,抗性较强。总体来看,RF1-12-5田间对亚洲玉米螟、棉铃虫抗性较高,对黏虫抗性略低;室内心叶期对亚洲玉米螟抗性较弱,但在其他时期对亚洲玉米螟、黏虫、棉铃虫均表现了较好的抗性。因此RF-12-5具有推广应用的潜力。更多还原

【Abstract】 Through filed experiments and indoor bioassay,the resistances of domestic transgenic maize hybride Ruifeng 1-Double Resistance 12-5(RF1-12-5) against three major maize lepidopteran pests,Pyrausta nubilalis,Mythimna separate and Helicoverpa armigera were studied. The results of the filed experiments showed that the resistance of RF1-12-5 against Ostrinia furnacalis and Helicoverpa armigera were in high level during the corresponding identification periods. The resistance against Mythimna separate was in moderate level during the whorl stage. The indoor bioassay results showed that RF1-12-5 had slower resistance against Ostrinia furnacalis at whorl stage. After feeding with RF1-12-5 leaves for 24,48 and 72 hours,the corrected mortality rates were respectively 63. 97%,77. 14% and 88. 00%. At filamentous stage,the resistance was significant and the corrected mortality rate reached 100% after 48 hours. During the grain period,the corrected mortality rates were respectively 68. 86%,83. 96% and 98. 03% after 24,48 and 72 hours. ForMythimna separate,at whorl stage,there was no obvious resistant effect after 24 hours,but the corrected mortality rates were 94. 64% and 100% respectively after 48 and 72 hours. For Helicoverpa armigera,RF1-12-5 showed obvious resistance at filamentous stage and the corrected mortality rates were 87. 59% and100% after 24 and 48 hours respectively. In all,RF1-12-5 showed obvious field resistance against Ostrinia furnacalis and Helicoverpa armigera,but lower resistance against Mythimna separate. It showed lower resistance against Ostrinia furnacalis at whorl stage during indoor bioassay,but better resistance against Ostrinia furnacalis,Mythimna separate and Helicoverpa armigera at other stages. We concluded that RF1-12-5 had the potential for popularization and application in the future.更多还原