【摘要】 Bax inhibitor-1(BI-1)是调控内质网胁迫(endoplasmic reticulum stress,ER stress)介导的细胞死亡的关键因子,在植物耐逆中具有重要作用。目前,棉花BI-1(GhBI-1)的耐逆功能及其调控细胞死亡的相关报道较少。在本研究中,采用ER stress专一性诱导毒素——衣霉素(tunicamycin,TM)对棉花幼苗进行胁迫处理,实时定量PCR结果表明,GhBI-1的TM诱导表达具有组织特异性,根GhBI-1对TM的响应更加强烈。通过TM抗性试验及细胞死亡的分析发现,GhBI-1的表达提高了拟南芥的TM抗性,减缓了ER stress介导的细胞死亡。此外,未折叠蛋白反应信号通路中的bZip60转录因子基因的表达受GhBI-1的调控。更多还原

【Abstract】 Bax inhibitor-1(BI-1) regulates cell death mediated by endoplasmic reticulum stress(ER stress),and plays important roles in plant stress tolerance. Up to now,the role of cotton BI-1(GhBI-1)involved in stress tolerance and cell death remain largely unknown. In the present study,we characterized the expression patterns of GhBI-1 under ER stress conditions induced by tunicamycin(TM). The results of real-time quantitative PCRs showed that the inducible expression pattern of GhBI-1 was tissue specific. The expression of GhBI-1 in roots is stronger compared with in leaves under ER stress conditions. The overexpression of GhBI-1 increased the TM resistance of transgenic Arabidopsis and delayed cell death mediated by ER stress. The analysis of the expression of b Zip60 involved in unfolded protein response showed that GhBI-1 activated the avtivity of b Zip60 and improved cell tolerance to ER stress.更多还原