【摘要】 以金纳米溶胶、银纳米溶胶和金-银合金纳米溶胶为基底测定了小鼠血清表面增强拉曼光谱（SERS）.观察了血清蛋白质结构的主链、侧链和二级结构的振动峰(958、1 003、1 015、1 146、1 352 cm^-1),以及血清中少量碳水化合物D-甘露醇和脂类的特征峰(527、1 469 cm^-1),并比较了3种金属溶胶基底的SERS增强效应.结果表明,以金-银合金纳米溶胶为基底的SERS谱峰都得到增强,特别是527、958、1 003、1 146、1 352、1 469 cm^-1处信号加强明显.以银纳米溶胶为基底的SERS中蛋白质结构的振动峰也有一定的增强,但是在527 cm^-1处只有一小峰,未见1 469 cm^-1峰.以金纳米溶胶为基底的SERS中蛋白质结构的振动峰也有所加强,但527、1 469、1 547 cm^-1峰消失.实验结果表明,金-银合金纳米溶胶的SERS活性最强,银纳米溶胶其次,金纳米溶胶最差.更多还原

【Abstract】 The surface-enhanced Raman spectroscopy（ SERS） in mice serum was determined with the gold,silver and Au-Ag alloy nano sol as substrates. The characteristic peaks at 958,1 003,1 015,1 146,1 352 cm^-1 which assigned to the main chain,side chain and secondary structure of serum protein were observed. And the vibrating peaks observed at 527,1 469 cm^-1 were the characteristic peaks of small amount of carbohydrate D-mannitol and lipid in serum. Moreover,the SERS enhancement effects of three kinds of metal sol substrate were compared. The results indicated that SERS peaks were enhanced by using Au-Ag alloy nano sol as substrate. Especially,the characteristic peaks were enhanced significantly at527,958,1 003,1 146,1 352,1 469 cm^-1. When using silver nano sol as substrate,the SERS characteristic peaks of protein structure had certain enhancement. But merely there was a small peak at 527 cm^-1,the peak of 1 469 cm^-1 was invisible. When using gold nano sol as substrate,the characteristic peaks of protein structure were also strengthened,but the peaks at 527,1 469,1 547 cm^-1 were disappeared. The results showed that the SERS activity of Au-Ag alloy nano sol was the strongest,silver nano sol was moderate,and gold nano sol was the weakest.更多还原