【摘要】 目的探讨检测男性不育症患者Y染色体微缺失的临床意义。方法引用多重聚合酶链反应(PCR)对门诊392例男性不育惠者行Y染色体AZFa、AZFb、AZFc、AZFd和SRY20个序列标签位点(STS)设计特异引物进行微缺失检测,同时进行精液常规检查。结果 392例男性不育患者中AZF基因微缺失患者共检出24例,总缺失率为6.12%,其中汉族无精子症组缺失率为6.19%,维吾尔族(以下简称维族)无精子症组缺失率为18.92%,汉族少精子症组缺失率为5.81%,维族少精子症组缺失率为16.67%,汉族弱精子症组缺失率为6.56%,维族弱精子症组缺失率为0%。汉、维族不育症患者Y染色体AZF微缺失率比较差异无统计学意义(P>0.05);汉、维族无精子症患者Y染色体AZF微缺失率比较差异有统计学意义(χ~2=5.333,P=0.021),汉族与维族的Y染色体多位点联合缺失发生率比较差异有统计学意义(χ~2=4.168,P=0.041);汉、维族少精子症患者Y染色体AZF微缺失率比较差异无统计学意义(P>0.05);汉、维族弱精子症患者Y染色体AZF微缺失率比较差异无统计学意义(P>0.05)。结论汉、维族不育症男性患者中Y染色体AZF微缺失发生率存在种族差异,PCR检测AZF基因是诊断Y染色体AZF微缺失的较好的方法。更多还原

【Abstract】 Objective:To study the detection of male infertility patients lack of Y chromosome micro clinical significance.Methods:Multiple polymerase chain reaction(PCR) reference for outpatient service 392 cases of male infertility patients Y chromosome AZFa,AZFb,AZFc,AZFd and SRY20 sequence tags sites(STS) design specific primers for missing detection,semen routine checks at the same time.Results:392 cases of male infertility patients AZF gene micro defect patients were detected in 24 cases,total loss rate was 6.12%,while the loss rate was 6.19%,no sperm group Uighur group(hereinafter referred to as the Uighur) no sperm loss rate was 18.92%,the Han nationality less sperm group loss rate was 5.81%,the Uighur less sperm group loss rate was 16.67%,the Han nationality weak sperm group loss rate was 6.56%,the lack of Uighur weak sperm group at a rate of 0%.Han and Uighur infertility patients with Y chromosome AZF micro missing rate differences had no statistical significance(P>0.05);Sperm Han and Uighur Y-chromosome AZF micro missing rate difference was statistically significant(chi-square=5.333,P=5.333),the absence of Y chromosome loci union of Han and Uighur rates compare the difference was statistically significant(chi-square=4.168,P=4.168).Han and Uighur sperm less Y-chromosome AZF micro missing rate differences had no statistical significance(P>0.05);Han and Uighur sperm weak Y chromosome AZF micro missing rate comparison difference has no statistical significance(P>0.05).Conclusions:In patients with Han and Uighur infertility male incidence of Y chromosome loss of AZF micro racial differences,PCR detection AZF gene is diagnosis Y-chromosome AZF micro lack good method.更多还原