【摘要】 目的建立HPLC法测定清热暗疮丸中绿原酸、栀子苷、穿心莲内酯和脱水穿心莲内酯的含量。方法色谱柱为Agilent Eclipse XDB-C₁₈（4.6 mm×150 mm,5μm）;流动相为乙腈（A）-0.1%磷酸水溶液（B）,梯度洗脱;流速为1.0 m L·min^-1;柱温:30℃;波长切换时间序列采样:0⁷ min为327 nm,7¹2 min为238 nm,12¹9 min为225 nm,19⁴0 min为254 nm。结果绿原酸、栀子苷、穿心莲内酯和脱水穿心莲内酯分别在0.188³.754μg（r=0.999 8）、0.164³.272μg（r=0.999 8）、0.020⁰.392（r=0.999 9）μg和0.039⁰.777μg（r=0.999 9）范围内线性关系良好。平均回收率分别为99.53%,RSD为1.7%（n=9）;99.33%,RSD为1.5%（n=9）;99.59%,RSD为1.8%（n=9）和99.46%,RSD为1.5%（n=9）。5批清热暗疮丸中绿原酸、栀子苷、穿心莲内酯和脱水穿心莲内酯含量分别为9.48,8.05,0.92,1.92 mg·g^-1。结论该方法准确、快捷、可靠,可更好地用于清热暗疮丸的质量控制。更多还原

【Abstract】 Objective To establish a HPLC method for simultaneous determination of chlorogenic acid,geniposide,andrographolide and dehydroandrographolide in Qingre Anchuang Pills. Methods Agilent Eclipse XDB-C18（4.6×150mm,5 μm）column was used. The mobile phase was acetonitrile（A）-water containing 0.1 % phosphoric acid（B）with gradient elution mode at the flow rate of 1.0 m L·min-1. The detection wavelength was 327 nm（0-7 min）for chlorogenic acid,238 nm（7-12 min） for geniposide,225 nm（12-19 min） for andrographolide,254 nm（19-40 min） for dehydroandrographolide. Results Chlorogenic acid had a good linearity in the range of 0.188-3.754 μg, and the average recovery was 99.53 % with RSD being 1.7 %（n=9）. Geniposide had a good linearity in the range of 0.164-3.272 μg,and the average recovery was 99.33 % with RSD being 1.5 %（n=9）. Andrographolide had a good linearity in the range of 0.020-0.392 μg, and the average recovery was 99.59 % with RSD being 1.8 %（n=9）.Dehydroandrographolide had a good linearity in the range of 0.039-0.777 μg,and the average recovery was 99.46 %with RSD being 1.5 %（n=9）. The contents of chlorogenic acid,geniposide,andrographolide and dehydroandrographolide in 5 batches of Qingre Anchuang Pills were 9.48,8.05,0.92,1.92 mg·g^-1respectively. Conclusion The established method is simple,accurate and reproducible. It can be used for the quality control of Qingre Anchuang Pills.更多还原