【摘要】 目的:探讨补肾活血中药对体外糖基化终末产物(AGEs)条件下视网膜Müller细胞活力的影响。方法:体外纯化培养7天SD大鼠视网膜Müller细胞至P2代;中药血清药理学方法制备补肾活血中药含药血清;体外纯化培养的P2代视网膜Müller细胞置于AGEs状态下,以补肾活血中药含药血清干预,检测乳酸脱氢酶(LDH)漏出量。结果:(1)低AGEs组较正常对照组LDH值有升高趋势,其差异无统计学意义(P>0.05);高AGEs组较正常对照组LDH值明显升高,其差异有统计学意义(P<0.05)。(2)正常中药干预组与正常对照组LDH值比较,其差异无统计学意义(P>0.05)。(3)AGEs中药干预组较AGEs组LDH值明显降低,其差异有统计学意义(P<0.05)。结论:AGEs条件可导致体外纯化培养的大鼠视网膜Müller细胞细胞膜稳定性下降,通透性增加,细胞活力降低,补肾活血中药含药血清可提高AGES条件下视网膜Müller细胞活力,这可能是补肾活血中药防治糖尿病性视网膜病变(DR)的药物干预途径之一。更多还原

【Abstract】 Objective: To investigate the effect of Bushen Huoxue formula on the activity of retinal Müller cells( RMCs)purified in vitro under AGEs conditions. Methods: The RMCs of seven-day SD rats was cultured by way of modified enzyme-digestion method. The serum was made from Bushen Huoxue formula. Purified RMCs were cultured under AGEs conditions,and the serum was added to the culture medium. After 48 hrs,LDH leakage was detected. Results:1. Compared to the normal control group,there was an increasing trend of LDH leakage in the low-dose AGEs group,but there was no significant difference between the two groups( Ρ > 0. 05); LDH leakage was increased significantly in the high-dose AGEs group compared to the normal control group( Ρ < 0. 05). 2. There was no statistical significance in terms of LDH leakage between the TCM intervention group and the normal control group( Ρ > 0. 05). 3. The LDH leakage of the TCM intervention group was reduced significantly compared to the AGEs group( Ρ < 0. 05). Conclusion:The serum made from Bushen Huoxue formula could increase the activity of RMCs under AGEs conditions,which may be its mechanism for diabetic retinopathy.更多还原