【摘要】 目的观察辣椒素对脑缺血再灌注模型大鼠的影响并对其机制进行初步探讨。方法将75只健康雄性SD大鼠随机分为5组:空白组、模型组、辣椒素高剂量组、辣椒素中剂量组和辣椒素低剂量组,每组15只。除空白组外,线栓法建立大鼠右脑缺血再灌注模型。辣椒素高、辣椒素中和辣椒素低剂量组分别灌胃辣椒素100、50、25 mg/(kg·d),其他两组灌胃相应剂量的CMC-Na溶液,连续7 d。随后进行神经功能学评分、干湿重法检测大鼠脑含水量、TUNEL法测定神经元的凋亡、Western blot法测定cleaved Caspase-3蛋白表达情况。结果辣椒素高、辣椒素中和辣椒素低剂量组均显著降低了神经功能评分、脑含水量、神经元凋亡数量和cleaved Caspase-3蛋白的表达(P<0.01)。结论辣椒素对脑缺血再灌注有一定的保护作用,其机制可能与抑制cleaved Caspase-3蛋白的表达有关。更多还原

【Abstract】 Objective: To study the effects of capsaicin on rats of cerebral focal ischemia with reperfusion and its mechanism. Methods: 75 SD rats were randomly divided into 5 groups: control group,model group,cap high dose,cap middle dose and cap low dose group with 15 rats in each group. The suture method was used to establish the model of MCAO-reperfusion except the control group. Cap high dose,cap middle dose and cap low dose group were given 100,50 and 25 mg/kg cap for consecutive 7 days. Neurological deficit was scored,brain water content was detected with dry-wet method,neuronal apoptosis was measured with TUNEL method and level of cleaved caspase-3 were detected with western blot method. Results: Neurological deficit testing scores,brain water content,neuronal apoptosis and the level of cleaved caspase-3 decreased significantly in cap high dose,cap middle dose and cap low dose group(P < 0.01) and the difference was statistically significant compared the control group. Conclusion: Capsaicin has a protective effect on the rats of cerebral focal ischemia with reperfusion,and its mechanism may be related to reducing the expression of cleaved Caspase-3 protein.更多还原