【摘要】 目的通过高效氯氰菊酯对大鼠血液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量影响的检测,探讨其可能的毒作用机制。方法将雄性Wistar大鼠随机分为4组,即高效氯氰菊酯高、中、低剂量组和对照组。经口灌胃30 d,观察大鼠一般状况及体重的变化,实验结束后腹主动脉采血检测血清中SOD、GSH-Px活力和MDA含量;取脑、肝、肾、脾脏、睾丸组织称重,计算脏器系数,部分组织放于10%甲醛中固定待做病理组织学检查。结果高效氯氰菊酯可引起大鼠体重降低,实验第4周体重高剂量组(281.43±15.87)g,中剂量组(295.55±14.60)g,低剂量组(304.81±14.78)g,对照组(315.29±17.62)g,染毒组分别与对照组比较,差异均有统计学意义(P<0.05);脑/体比和睾/体比增加,高剂量组为(0.47±0.03)、(1.17±0.14),中剂量组为(0.46±0.03)、(1.20±0.11),低剂量组为(0.45±0.02)、(1.12±0.07),对照组为(0.43±0.03)、(1.08±0.11),染毒组分别与对照组比较,差异均有统计学意义(P<0.05);随着染毒剂量的增加,大鼠血清中SOD、GSH-Px活力逐渐降低,高剂量组为(34.57±1.83)U/ml、(880.83±55.73)μmol/L,中剂量组为(43.69±1.83)U/ml、(1 014.18±41.69)μmol/L,低剂量组为(54.42±1.82)U/ml、(1 129.72±31.17)μmol/L,对照组为(65.46±1.53)U/ml、(1 177.62±54.89)μmol/L;而MDA含量逐渐升高,高剂量组为(6.35±0.45)nmol/ml,中剂量组为(4.92±0.35)nmol/ml,低剂量组为(3.67±0.43)nmol/ml,对照组为(2.40±0.57)nmol/ml,染毒组分别与对照组比较,差异均有统计学意义(均P<0.05)。结论氧化性损伤可能是高效氯氰菊酯毒作用机制之一。更多还原

【Abstract】 [Objective]To detect the effect of beta-cypermethrin on activities of Superoxide Dismutase(SOD),Glutathione peroxdase(GSH-Px)and content of Maleic Dialdehyde(MDA)in blood of rats,explore its possible toxic mechanism.[Methods]Male Wistar rats were randomly divided into 4 groups,including beta-cypermethrin low dose group,middle dose group,high dose group and control group. The rats were treated by gavage for 30 days,and the general condition and body weight changes were observed.At the end of experiment,the blood samples were collected from abdominal aorta,to detect the activities of SOD and GSH-Px and content of MDA. The brain,liver,kidney,spleen and testis tissues were weighed to calculate the organ coefficients,and some tissues were fixed in 10% formalin to conduct the histopathological examination.[Results]Beta-cypermethrin caused weight loss in rats. The weight of animals in high dose group,middle dose group,low dose group and control group were respectively(281.43±15.87 g),(295.55±14.60 g),(304.81±14.78 g)and(315.29±17.62 g),and the differences were statistically significant between exposure groups and control group(P<0.05). The brain-to-body mass ratio and testicular-to-body ratio increased,those in high dose group,middle dose group,low dose group and control group were respectively(0.47 ±0.03,1.17 ±0.14),(0.46 ±0.03,1.20 ±0.11),(0.45±0.02,1.12±0.07)and(0.43±0.03,1.08±0.11),and the differences were statistically significant(P<0.05). With the increase of exposure dose,the activities of SOD and GSH-Px in serum decreased gradually,those in high dose group,middle dose group,low dose group and control group were respectively(34.57 ±1.83 U/ml,880.83 ±55.73 μmol/L),(43.69 ±1.83 U/ml,1 014.18 ±41. 69 μmol/L),(54.42±1.82 U/ml,1 129.72±31.17 μmol/L)and(65.46±1.53 U/ml,1 177.62±54.89 μmol/L),while MDA content gradually increased,that in high dose group,middle dose group,low dose group and control group was respectively(6.35±0.45 nmol/ml),(4.92±0.35 nmol/ml),(3.67±0.43 nmol/ml)and(2.40±0.57 nmol/ml),and the differences were statistically significant(P<0.05).[Conclusion]Oxida-tive injury may be one of the mechanisms of toxic action of beta-cypermethrin.更多还原