【摘要】 目的:建立脾胃虚弱型肥胖小鼠模型并进行评价。方法:复制高脂饮食诱导肥胖模型,在肥胖模型的基础上按照神疲乏力、食少纳呆、便溏等主要脾虚指标进行二次筛选符合条件的肥胖鼠。通过ELISA法检测血清总胆固醇、甘油三酯、高密度脂蛋白及低密度脂蛋白含量,测定血清中炎症因子(白介素-6、白介素10及肿瘤坏死因子-α)及脾脏、胸腺和肠道派氏小体中CD4~+、CD8~+及CD11c~+等含量变化。结果:肥胖鼠成模率62.96%,其中脾胃虚弱型肥胖鼠23.53%。脾虚型肥胖鼠IL-10、TNF-α显著高于正常组,白介素-6显著低于正常组,其肠道派氏小体中的CD4~+、CD8~+及CD11c~+明显降低。结论:此方法可以较好地筛选出脾胃虚弱型肥胖鼠,为健脾益气治疗肥胖奠定基础。更多还原

【Abstract】 Objective: To copy and evaluate the spleen deficiency syndrome type in obese mice. Method: Replicating obese mice with high-fat diet,and on this basis,according to the spleen deficient fatigue,less food,stay,loose stools and secondary screening eligible obese mice,and through the ELISA method to detect serum total cholesterol,triglycerides,high-density lipoprotein and low density lipoprotein content,observe serum inflammatory factors( interleukin 6,interleukin10 and tumor necrosis factor alpha) and CD4+,CD8+,CD11c+content changes of the spleen,thymus and intestinal corpuscle. Results: The model of obesity mice was 62. 96%,23. 53% of which was spleen deficiency syndrome type. The IL-10,the TNF alpha of these mice is significantly higher than normal group,interleukin-6 was significantly lower than normal group. The CD4+,CD8+and CD11c+of their gut peyer’s patch were also significantly reduced. Conclusion: This method could better screen spleen deficiency syndrome by obesity animal medel.更多还原