【摘要】 目的:探讨金钗石斛多糖对肝纤维化大鼠转化生长因子β1(TGFβ1)、基质金属蛋白酶抑制因子(TIMP-1)及基质金属蛋白酶(MMP13)的影响。方法:SD大鼠按随机数字表法分为正常对照组,模型组,秋水仙碱组,扶正化瘀组,石斛多糖低、中、高剂量组,采用四氯化碳(CCL4)复合乙醇诱导大鼠肝纤维化模型,造模8周成功后,分别给予相应的干预药物,正常组、模型组给予生理盐水,每日1次,连续4周。采用荧光定量PCR技术检测大鼠肝脏TGFβ1、TIMP-1、MMP13 mRNA的表达差异。结果:与模型组相比,各治疗组肝组织内纤维化程度均有不同程度减轻;与正常对照组比较,模型组TGFβ1、TIMP-1 mRNA的表达上调(P<0.05),MMP13 mRNA的表达下调(P<0.05);与模型组比较,各药物干预组均能降低大鼠肝组织TGFβ1、TIMP-1 mRNA的表达,其中扶正化瘀组、石斛多糖中、大剂量表达有显著性差异(P<0.05);与模型组比较,各药物干预组提高MMP13 mRNA的表达,其中以扶正化瘀组、石斛多糖大剂量为甚(P<0.05)。结论:石斛多糖具有下调TGFβ1、TIMP-1 mRNA表达,上调MMP13 mRNA表达,可能是其抗肝纤维化的作用机理之一。更多还原

【Abstract】 Objective:To study the influence of transforming growth factor-β1( TGF-β1),tissue inhibitor of metalloproteinases-1( TIMP-1) and matrix metalloproteinase( MMP-13) in dendrobium nobile polyose on hepatic fibrosis rats.Methods:SD rats were randomly divided into normal group,model group,colchicine group,Fuzheng Huayu group,caulis dendrobii low-dose group,caulis dendrobii medial-dose group,caulis dendrobii high-dose group.SD rat models of hepatic fibrosis were induced by carbon tetrachloride composite ethanol( CCl4).After 8 weeks,each group was given corresponding drug intervention respectively.The normal group and model group were given normal saline once a day for 4 weeks.Use fluorescence quantitative PCR technique to detect mRNA expression differences of TGF-β1,TIMP-1and MMP-13 in liver.Results:Compared with normal group,the mRNA up-regulated expressions of TGF-β1 and TIMP-1( P < 0.05) and the mRNA down-regulated expression of MMP-13( P < 0.05) in model group.Compared with model group,the each drug intervention group can reduce mRNA expressions of TGF-β1 and TIMP-1.The expressions in Fuzheng Huayu group,caulis dendrobii medial-dose group and caulis dendrobii high-dose group had significant differences( P < 0.05).Compared with model group,each drug intervention group can improve mRNA expression of MMP-13,especially in Fuzheng Huayu group and caulis dendrobii high-dose group( P < 0.05).Conclusion:The dendrobium nobile polyose can reduce mRNA expressions of TGF-β1 and TIMP-1 and improve mRNA expression of MMP-13.It may be one of anti-hepatic fibrosis mechanisms.更多还原