【摘要】 目的:探讨基因Ⅲ型戊型肝炎病毒(HEV)非结构蛋白开放阅读框3(ORF3)对体外培养的人肝癌PLC/PRF/5细胞中Ⅰ型干扰素(IFN-α和IFN-β)及干扰素刺激基因(ISG)15蛋白表达的影响。方法:分别用基因Ⅲ型戊型肝炎病毒ORF3质粒及空载质粒转染PLC/PRF/5细胞,在转染之后不同时间点(0h,8h,12h,24h,48h,72h,120h),用ELISA方法检测细胞上清液中Ⅰ型干扰素的含量,并用Western blot方法检测ISG15蛋白的表达。结果:ORF3组细胞上清液中IFN-α、β的水平均比空载组及空白组升高,而空载组与空白组之间无明显改变。并且ORF3组细胞中ISG15蛋白的表达增加,而空载组与空白组无明显改变。结论:基因Ⅲ型HEV的非结构蛋白ORF3促进Ⅰ型干扰素(IFN-α和IFN-β)及ISG15蛋白的表达。更多还原

【Abstract】 Objective:To study the effect of.genotype 3 hepatitis E virus ORF3 protein onthe production of type I interferon and ISG15 protein in Human hepatocellular carcinoma cells.Methods:Hepatocellular carcinoma cell line PLC-PRF-5 was respectively transfected with the genotype Ⅲ hepatitis E virus ORF3 plasmid and the empty plasmid.At different time points after transfection(0h,8h,12 h,24h,48 h,72h,120h),the production of IFN I in supernatants was measured by ELISA and the expression of ISG15 protein was assayed by Western blot.Results:The levels of IFN α and β in the ORF3 plasmid groupsupematant were obviously higher than those in the empty plasmid group and blank group,but there was no significant change in the empty plasmid group and blank group.And the expression of ISG15 protein was increased in ORF3 plasmid group,but there was no significant change in the empty plasmid group and blank group.Conclusion:Genotype Ⅲ HEV ORF3 protein promotes the expression of type Ⅰ interferon(IFN α and IFN β) and ISG15 protein.更多还原