【摘要】 目的探讨噬血细胞综合征患儿外周血Th17、Th22及Treg细胞频数及相关细胞因子变化在HPS发生发展中的作用。方法收集本院自2014年收治的HPS初发患儿68例,其中缓解患儿55例,死亡13例,以同期健康体检儿童30例作为对照组,以流式细胞仪检测3组外周血Th17、Th22细胞及Treg细胞频数,以酶标仪检测患儿血浆中细胞因子IL-17、IL-21、IL-22及IL-35。结果 HPS初诊组外周血Th17、Th22细胞比例及其相关细胞因子IL-17、IL-21、IL-22较对照组、HPS缓解组均明显升高,差异均有统计学意义(P<0.05),而Treg细胞频数及其相关细胞因子IL-35较对照组、HPS缓解组均明显降低,差异有统计学意义(P<0.05)。结论 Th22、Th17细胞及其相关细胞因子IL-22、IL-17及IL-21在HPS患儿外周血中增高,而Treg细胞及其相关细胞因子IL-35比例下降,在HPS的发生发展中可能起着重要作用。更多还原

【Abstract】 Objective To explore the changes of frequencies of Th17,Th22 and Treg cells and changes of their related cytokines in the peripheral blood of Hemophagocytic syndrome( HPS) patients in the development of HPS. Methods 68 patients with incipient HPS admitted in our hospital from 2014 were collected,including 55 patients with remission,13 cases of death.At the same time,30 cases accepted physical examination with physical fitness as control group. The frequencies of Th17 cells,Th22 cells and Treg cells in the peripheral blood of the 3 groups were tested by flow cytometry instrument; related cytokines including IL-17,IL-21,IL-22 and IL-35 by ELISA in plasma of patients with HPS were tested. Results The proportion of Th17 cells,Th22 cells and their related cytokines( IL-17,IL-21 and IL-22) raised obviously than patients in control group and ease group,and the differences were statistically significant( P < 0. 05),while frequencies of Treg cells and their related cytokines IL-35 of patients in ease group were significantly decreased in the incipient group than control group and ease group,and the differences were statistically significant( P < 0. 05). Conclusion Frequencies of Th22,Th17 cells and their related cytokines( IL-17 and IL-22) raised in the peripheral blood of HPS patients,while frequency of Treg cell and its related cytokines IL-35 declined,which may play an important role in the development of the occurrence of HPS.更多还原