【摘要】 目的应用核酸适体9-3-35和硫黄素T,建立放射性惰性气体氡的非标记荧光传感分析新方法。方法用硫磺素T诱导富G序列核酸适配体9-3-35形成稳定的G-四链体结构,产生荧光信号,采集氡辐射得到稳定的子体铅,加入Pb²⁺后导致体系荧光信号敏锐变化,灵敏检测氡。结果 Pb²⁺离子浓度为1.0 nmol/L¹25.0 nmol/L时,荧光强度改变量（ΔF）与Pb²⁺离子浓度呈现良好的线性关系,ΔF=9.13cPb+163.40,r=0.995 3;氡累积浓度为3.0×104Bq·h/m³².2×105Bq·h/m³时,ΔF值与氡累积浓度之间的数学定量模型为ΔF=35.59cRn+229.51,r=0.994 0。新方法对铅的检出限为0.312 nmol/L,对氡的检出限为1.87×103Bq·h/m³,低于国家标准方法中氡的累积测量的检出限（2.1×103Bq·h/m³）。结论本方法可以灵敏检测氡,在氡的采样和检测过程中避免了辐射危害;可直接测定氡样品,操作简单,成本低廉,拓展了核酸适体对放射性物质和气体的荧光传感检测新领域。更多还原

【Abstract】 Objective To establish a new label-free fluorescence method for the determination of radioactive inert gas radon by using the aptamer 9-3-35 and Thioflavine T. Methods Using Thioflavine T induced G rich nuclei acid sequence 9-3-35 to form stable conformation G-quadruplex structure,which produced fluorescence signal. Collecting stable daughter lead from radon radiation,after the Pb²⁺was added to the system,a sharp fluorescence signal change emerged,then the radon was sensitive detected. Results When the concentration of Pb²⁺concentration was within 1. 0 nmol/L-125. 0 nmol/L,the fluorescence intensity change（ΔF） and Pb²⁺concentration showed a good linear relationship,ΔF = 9. 13cPb+ 163. 40,r = 0. 995 3;When the radon concentration was within 3. 0 × 104Bq·h/m³-2. 2 × 105Bq·h/m³,ΔF = 35. 59cRn+ 229. 51,r = 0. 994 0.The detection limit of the new method for lead ions was 0. 312 nmol/L and that of radon was 1. 87 × 103Bq·h/m³. This method was far below the detection limit for cumulative radon measurement from the Chinese national standard method（2. 1 × 103Bq·h/m³）.Conclusion This sensitive method can exclude radiation damage in field testing. Furthermore,it expands a new fluorescence sensing detection field of aptamers for radioactive substances and gases.更多还原