【摘要】 目的探讨Th17/Treg细胞及其细胞因子与宫颈癌临床分期关系。方法选择本院2014年1月-2016年12月收治的宫颈癌患者60例,其中Ⅰ期15例、Ⅱ期20例、Ⅲ期12例、Ⅳ期13例。采用流式细胞术检测外周血Th17/Treg细胞表达频率,ELISA法检测IL-17和IFN-γ表达水平。结果 Th17细胞和Treg细胞百分率随临床分期增加而增加,其中Ⅰ期<Ⅱ期<Ⅲ期<Ⅳ期。IL-17表达水平随临床分期增加而增加,其中Ⅰ期<Ⅱ期<Ⅲ期<Ⅳ期。经Pearson相关分析,外周血Th17细胞百分率和细胞因子IL-17的表达水平与FIGO分期呈正相关(r=0.746,r=0.714,P<0.01)。Treg细胞百分率和IFN-γ表达水平与FIGO分期无相关性。结论宫颈癌患者外周血中Th17/Treg细胞的高表达参与了宫颈癌的发生、发展过程,有可能作为宫颈癌预后评估的新指标。更多还原

【Abstract】 Objective To investigate the relationship between the Th17/Treg cells and their cytokines and clinical stages of cervical cancer. Methods 60 patients with cervical cancer admitted in our hospital from January,2014 to December,2016 were enrolled,including 15 cases in stageⅠ,20 cases in stageⅡ,12 cases in stage Ⅲ and 13 cases in stage Ⅳ. The frequencies of Th17/Treg cells were detected by flow cytometry,and the expression of IL-17 and IFN-γ were detected by ELISA in the peripheral blood. Results The percentages of Th17 cells and Treg cells increased along the asending of clinical stages,including stageⅠ < stageⅡ < stage Ⅲ < stage Ⅳ. The expression of IL-17 increased along the asending of clinical stages,including stageⅠ < stageⅡ < stage Ⅲ < stage Ⅳ,and the expression of cytokine IL-17 was positively correlated with FIGO staging( r =0. 746,P < 0. 01; r = 0. 714,P < 0. 01) by correlation analysis of Pearson test; the percentages of Treg cells and the level of IFN-γ were not correlated with FIGO staging. Conclusion The high expression of Th17 cells in peripheral blood of patients with cervical cancer perhaps involves in the development and progression of cervical cancer. It may be a new biomarker of the prognosis of cervical cancer.更多还原