A putative parasitism gene named Mj-1-1(KU358725) was cloned based on the EST library of Meloidogyne javanica.The full length cDNA of Mj-1-1 is 573 bp,containing a 450 bp open reading frame(ORF).The ORF encodes 149 amino acids.Two introns(20 bp and 111 bp) exist in the 740 bp DNA sequence.NCBI Blastn showed that Mj-1-1 had a 97%similarity with a sequence(JQ284068) of Meloidogyne incognita.In situ hybridization showed that Mj-1-1 was expressed in the dorsal gland cell of M.javanica.The highest expression lev...