【摘要】 本文旨在从肉牛阴道中分离筛选益生乳酸菌,并对其功能进行评价,为开发微生态制剂治疗生殖道感染疾病奠定基础。采用传统培养方法从肉牛阴道中分离乳酸菌,并对其抑菌能力、抑菌物质、产酸能力和抗生素敏感性进行检测,通过16S r DNA序列分析方法对乳酸菌分离株进行鉴定。结果表明,通过体外试验获得4株乳酸菌,坚强肠球菌Y1抑菌能力最强,乳酸杆菌Y5的产酸能力强于其他菌株,4株乳酸菌均对氨苄西林和万古霉素敏感。研究结果表明,4株乳酸菌可作为潜在的益生菌株治疗母牛生殖道感染疾病。更多还原

【Abstract】 The aim of this study was to isolate and evaluate the probiotic properties of lactic acid bacteria from beef cattle for developing probiotics to treat infection of genital tract.The lactic acid bacteria were isolated by traditional culture method,and the lactic acid bacteria isolates were tested for anti-bacterial activity,anti-bacteria substance,the acid producing ability and antibiotics sensitivity.The lactobacillus isolates were identified by 16 S r DNA sequencing analysis.The results showed that 4 strains were obtained from in vitro test.Enterococcus durans Y1 had the best ability to inhibit the growth of pathogenic bacteria,and Lactobacillus aqilis had best ability to produce acid than other strains.All lactic acid bacteria were susceptive to Ampicillin and Vancomycin.These results show that 4 lactic acid bacteria could be used as candidates to treat infection of genital tract.更多还原