【摘要】 采用Western blot法检测犏牛、牦牛睾丸组织中DNA甲基转移酶3a(Dnmt3a)蛋白的相对表达量,用甲基化定量试剂盒(荧光法,Epigentek公司)检测其全基因组DNA甲基化的水平,分析犏牛与牦牛在这2个表达量上的差异。结果显示:犏牛、牦牛睾丸组织中Dnmt3a蛋白的相对表达量分别为(0.853±0.015)和(0.651±0.016),犏牛的显著高于牦牛(P<0.05);犏牛、牦牛睾丸组织的全基因组DNA甲基化水平分别为(0.950±0.013)%和(0.808±0.016)%,犏牛的显著高于牦牛(P<0.05)。可见,犏牛睾丸组织中Dnmt3a蛋白表达水平与其全基因组DNA甲基化水平均显著高于牦牛,这可能是犏牛雄性不育的DNA甲基化机制之一,也将为犏牛雄性不育的表观遗传学研究奠定基础。更多还原

【Abstract】 The objective of this study was to explore the possible mechanisms on DNA methylation in male sterility of cattle-yak.The expression levels of Dnmt3 aprotein were detected by Western blot in cattle-yak and yak testicular tissue and the levels of genome-wide DNA methylation were detected by fluorescence method using MethylFlash^TM Methylated DNA Quantification Kit（Epigentek company）in cattle-yak and yak testicular tissue.The results showed that the relative quantitative expression of Dnmt3 aprotein in cattle-yak and yak testicular tissue was（0.853±0.015）,（0.651±0.016）separately,that of cattle-yak was significantly higher than yak（P<0.05）.The levels of genome-wide DNA methylation in cattle-yak and yak testicular tissue were（0.950±0.013）%,（0.808±0.016）% separately,that in cattle-yak was significantly higher than yak（P<0.05）.In conclusion,the expression levels of Dnmt3 aprotein and genome-wide DNA methylation in cattle-yak testicular tissue were significantly higher than those in yak.It may be one of mechanisms on DNA methylation in male sterility of cattle-yak,which will lay the foundation for studies of epigenetics in male sterility of cattle-yak.更多还原