【摘要】 【目的】研究骨桥蛋白(OPN)基因启动区多态性与乙型肝炎病毒相关肝细胞癌(HBV-HCC)遗传易感性的关系。【方法】225例确诊为HBV-HCC患者与200例年龄匹配的慢性乙型肝炎患者被纳入研究。OPN基因启动区3个多态性位点(-156del G/G,-443T/C和-616T/G)通过直接测序法确定基因型。【结果】HBV-HCC组-156 del G/del G基因型分布频率高于对照组(P=0.003)。HCC组-156 del G等位基因频率显著增高(P<0.001)。Logistic回归分析显示等位基因-156del G是HBV-HCC的易感因素(OR=1.643,95%CI 1.249-2.161)。两组间-443T/C、-616T/G基因型分布和等位基因频率差异没有统计学意义。【结论】OPN基因启动区-156等位基因可能是中国人群HBV-HCC的易感因素。更多还原

【Abstract】 【Objective】To study the association between the polymorphisms in the promoter region of Osteopontin(OPN)with hepatitis B virus(HBV)-related HCC. 【Methods】A total of 225 cases diagnosed with hepatitis B virus(HBV)-related HCC and200 age-matched patients with HBV infection without HCC were collected. Three polymorphisms(-156 del G/G,-443T/C and-616T/G)in the Osteopontin promoter were genotyped using direct sequencing.【Results】The frequency of-156 del G/del G genotype in the HCC group was higher than that of in the control group(P = 0.003). There was a significantly increased frequency of the allele-156 del G(P < 0.001)in HCC patients. Logistic regression analysis was performed to show an increase HCC risk associated with the del G variant genotype(OR1.64;95%CI 1.25 ~ 2.16). There were no differences between the groups in the genotype distributions and allele frequencies of SNP-443T/C and-616T/G.【Conclusion】Our findings suggest that allele-156 del G in the Osteopontin promoter may be a marker for risk of HCC with HBV infection in Chinese Han population.更多还原