【摘要】 本试验考察了土壤温度、有机质含量和pH对海洋生防细菌多粘类芽胞杆菌L₁-9在黄瓜根表土壤中定殖的影响。采用实时荧光定量PCR方法检测了不同时期黄瓜根表土壤中菌株L₁-9的16S r DNA拷贝数。结果表明,土壤有机质含量、土壤温度和pH对菌株L₁-9在黄瓜根表的定殖有显著影响（P<0.01）。25℃定殖数量最高,为5.79×108拷贝/g土,是15℃时的3.4倍;添加有机肥20⁴0 g/kg土壤,可以促进菌株L₁-9在黄瓜根际定殖;pH 7时,菌株L₁-9在黄瓜根表土壤中的定殖数量最高。本研究为海洋生防菌L₁-9防治黄瓜土传病害提供了理论依据。更多还原

【Abstract】 In this study, the effects of soil organic matter contents, temperature and soil pH value on colonization of marine biocontrol bacterium of L₁-9 in root surface soil were investigated. 16 S r DNA gene fragments of the strain in the root surface soil were quantified using quantitative real-time PCR method. The results showed that soil organic matter content, temperature and pH exhibited significant impacts on colonization of the strain L₁-9 in root surface soil（P<0.01）. The propagation of the strain L₁-9 in root surface soil was faster under the temperature of 25 ℃, which was 5.79×108 copies per gram dry soil, and 4.3 times of that under 15 ℃. Addition of organic matter（20―40 g/kg） increased the population density of the strain L₁-9. The maximum colonization level of the strain L₁-9 in the root surface soil was achieved in pH 7. This study laid a foundation for the development and application of the marine biocontrol bacterium L₁-9 against soilborne diseases of cucumber.更多还原