【摘要】 目的分析缺血性脑卒中(IS)患者恢复稳定期外周血单个核细胞(PBMCs)中的差异表达基因,为探讨脑卒中恢复稳定期的遗传病理机制提供生物信息学线索。方法选择GEO数据库中GDS4521芯片数据,该芯片以年龄、性别相匹配的20例IS恢复稳定期(脑卒中发生至少6个月以上)患者和20例未发生过脑卒中者作为研究对象,收集其PBMCs进行基因芯片检测,利用GEO2R、DAVID、g:profiler和String等工具,筛选和分析差异表达基因功能富集和相关信号通路情况。结果脑卒中恢复稳定期组与对照组相比,在PBMCs中发现37个基因表达明显变化,其中34个上升,3个下降。GO分析表明,这些差异表达基因在生物过程方面,以炎性反应、中性粒细胞趋化相关基因为最多;在分子功能方面,以趋化因子活性相关基因为最多。KEGG信号通路分析表明,位于TNF信号通路中的差异表达基因数量最多。相互作用网络图揭示,这些差异基因主要以与炎性反应相关的两个网络为主。结论 IS发生超过6个月后仍有多种功能蛋白和信号通路可能发生改变,特别是与炎性反应相关的蛋白和信号通路,提示炎性反应在IS的恢复稳定期仍可能对疾病的预后和再发起作用。更多还原

【Abstract】 Objective To screen differential expression genes(DEGs)in peripheral blood mononuclear cells(PBMCs)of patients with ischemic stroke(IS)during convalescent phase,and to analyze their functional characteristics,in order to further understand the pathologic mechanism of stroke and provide some bioinformatic clues.Methods The data of Chip GDS4521 collected from PBMCs in the convalescent phase of IS were chosen from the GEO database.GEO2 Ranalysis tools were used to search for DEGs,and the characteristics of gene function enrichment and related signal pathways in these DEGs were analyzed by DAVID,g:profiler and String.Results Compared with the normal control group,the expression of 37 genes in PBMCs was significantly changed in the IS recovery period(> 6months),34 of which were significantly increased and 3genes decreased significantly.Gene Ontology(GO)analysis showed that in biology process these DGEs were mainly involved in inflammatory process,neutrophil chemotaxis,cell proliferation and apoptosis,and signal transduction.In molecular function most of them are related to chemokine activity and receptor binding,transcription factor activity,cytokine activity,and so on.KEGG signaling pathway analysis showed that the DEGs were mainly involved in TNF,NF-κB,chemokines,TOLL-like receptors and NOD-like receptors signaling pathways.The network map of the DEGs revealed that these genes were mainly dominated by two network maps related to inflammation.Conclusions This study demonstrated that many functional proteins and multiple signaling pathways may change at least 6 months after IS,especially in inflammation-related proteins and pathways,suggesting that inflammatory response might play a key role not only in acute but also in convalescent phase of IS.更多还原