【摘要】 目的了解不同宿主来源的肺炎克雷伯菌耐药性和分子特点,探讨其在人与动物间传播的可能性。方法收集2013年3月至2014年12月肺炎克雷伯菌98株(包含动物源67株、医源31株),通过K-B纸片扩散法和肉汤稀释法测定对15种抗菌药物的敏感性;拉丝试验测定产粘液表型;PCR扩增2个毒力基因和7个耐药基因;多位点序列(MLST)分析分子型。结果医源菌株耐药率显著高于动物源菌株,在动物源菌株中,鸡源和猪源菌株耐药率高于兔源和犬源菌株;除犬源菌株外均表现多重耐药,医源菌株多重耐药率最高(74.19%)。98株肺炎克雷伯菌有18个ST型,鸡源菌株主要流行ST37、猪源菌株为ST258、兔源菌株为ST60、犬源菌株为ST11,医源菌株为ST11。ST11为医源、犬源、猪源菌株共有,ST235为医源和鸡源菌株共有,ST258为医源和猪源菌株共有。高粘液菌株主要为ST11、ST235、ST258,且在这些分子型中检测到rmpA、magA基因。医源菌株blaKPC的检出率最高(54.84%),犬源和兔源菌株未检出BlaKPC基因。超广谱β-内酰胺酶基因在医源菌株中检出率高于动物源菌株,qnrA和qnrB基因在鸡源菌株中检出率高于医源菌株。ST11、ST258、ST235携带的多种耐药基因最高。结论不同宿主来源的肺炎克雷伯菌耐药表型、粘液表型及分子特性不同,但ST11、ST235、ST258为人源和动物源肺炎克雷伯菌共有的分子型。更多还原

【Abstract】 We aimed to detect the drug-resistant and molecular characteristic of Klebsiella Pneumoniae(KP)isolated from different hosts origin,and to investigate the infection possibility between animals and humans.A total of 98 KP strains were collected in Henan Province from March 2013 to December 2014.Drug-resistance to 15 antibiotics was detected by K-B diffusion methods.The phenotype of produce mucus was determined by stringing test.Seven drug-resistant gene and 2 virulence gene were amplified by PCR technique.Molecular types were analysis by MLST.The resistant rate of KP isolated from nosocomial was higher than those isolated from animals.Among animal strains,the resistant rate of KP isolated from chickens and pigs were higher than that from rabbits and dogs.The multidrug resistant(MDR)of nosocomial isolates were the highest(74.19%).There were 18 STs among 98 KP strains.The main prevalent types were ST37 in chicken,ST258 in pigs,ST60 in rabbits,ST11 in dogs and nosocomial respectively.ST11 was common epidemic types among nosocomial,dogs and pigs.ST235 was common molecular types among chicken and human.ST258 existed in both nosocomial and pigs.The rmpA gene and magA gene were detected in ST11,ST235 and ST 258,producing higher mucus.The blaKPCgene was 54.84%in nosocomial strains,but not been detected in dog and rabbit strains.The distribution of ESBLs gene in nosocomial was higher than those in animals,but qnrA gene and qnrB gene were higher in chicken than in human.The multidrug resistant gene was the highest distribution in ST11,ST258 and ST235.There were some differences in phenotype of drug-resistance,producing mucus and molecular characteristics,but ST11,ST258 and ST235 were common types of KP isolates from different host origins.更多还原