【摘要】 目的掌握上海市蜱分布及携带新型布尼亚病毒情况,了解上海市蜱动物宿主以及动物感染病毒状况,从而评估上海地区是否存在新型布尼亚病毒的传播风险。方法采用布旗法捕捉室外"游离蜱",动物"寄生蜱"采用动物体表采集法捕获犬体表蜱类样本;蜱病毒核酸的检测采用荧光定量PCR法;动物血清抗新型布尼亚病毒的总抗体检测采用双抗原夹心法。结果 2012—2014年期间,上海松江辰山和金山大金山岛监测到室外"游离蜱",蜱种均为长角血蜱;上海17个区中10个区监测到"寄生蜱",主要分布在崇明、奉贤、闵行等区,血红扇头蜱为优势蜱种。检测的143只蜱中,未发现病毒核酸。上海"寄生蜱"的动物宿主主要为犬,其次为羊,分布在6个主城区的198只犬、浦东新区和奉贤区的120份猪和崇明区的36头羊中,均未发现感染病毒。结论 2012—2014年上海地区蜱尚未发现新型布尼亚病毒的携带,动物监测中未发现感染。因此,尚无证据证实上海地区为新型布尼亚病毒自然疫源地,需进一步调查研究证实。更多还原

【Abstract】 To investigate the distribution of ticks and to detect the new bunyavirus in ticks and infection rate in animals in Shanghai,outdoor free ticks were captured by flag method and ticks on animals were collected by animal capture.SFTSV in ticks were detected by real-time RT-PCR.Total antibodies against SFTSV in host animal sera were tested by double antigen sandwich method.Results showed that during 2012-2014,free ticks were captured in Chenshan Park and Jinshan Island,which were all Haemaphysalislongicornis.Ten of seventeen districts found ticks on animals which were dominantly Rhipicephalus sanguineus.No nucleic acid of SFTSV was detected in 143 ticks.Main host animal for ticks was dog,sheep was in the second.Sera in 198 dogs from 6 urban districts,120 swine from Pudong and Fenxian districts and 36 sheep from Chongming District were all SFTSV antibody negative.Ticks were not found with SFTSV in Shanghai during 2012-2014.No SFTSV infection was found in host animals.Therefore,there is no evidence that Shanghai is the natural foci of SFTSV.Further surveillance and investigations should be carried out in the future.更多还原