【摘要】 目的探索白念珠菌CDR1/CDR2和MDR1基因高表达对氧化应激的影响。方法以白念珠菌CDR1/CDR2和MDR1基因高表达株及其亲本株为研究对象,测定过氧化氢(H2O2)对菌株生长的影响。以H2O2建立氧化应激模型,比较各菌株细胞内活性氧(Reactive oxygen species,ROS)、线粒体膜电位(Mitochondrial membrane potential,ΔΨm)、氧化应激相关基因(CAP1和GRP2)和ROS清除相关基因(SOD2和SOD5)转录水平的差异。结果各菌株均在5mmol/L H2O2作用时出现100%生长抑制;H2O2能升高细胞内ROS和降低ΔΨm(P<0.05),其中高表达株中两者的变化幅度较亲本株低(P<0.05);同时高表达株中CAP1和GRP2基因在转录水平的表达上调以及SOD2和SOD5基因的下调也较亲本株少(P<0.05)。结论 CDR1/CDR2和MDR1基因高表达能够降低白念珠菌的氧化应激反应,增强其对氧化应激的适应性。更多还原

【Abstract】 To investigate the effect of CDR1/CDR2 or MDR1 genes overexpression on oxidative stress in Candida albicans,we evaluated the effect of H₂O₂ on cell viability in C.albicans overexpressing genes CDR1/CDR2 or MDR1 and their parent strains.After establishing an oxidative stress model with H₂O₂,we detected reactive oxygen species（ROS）,mitochondrial membrane potential（ΔΨm）and the expression of oxidative stress response-related genes（CAP1and GRP2）and ROS clearance related-genes（SOD2and SOD5）.The results showed that C.albicans growth were inhibited by 100% after the treatment of 5mmol/L H₂O₂.H₂O₂ caused more ROS accumulation andΔΨm reduction in parent strains than in CDR1/CDR2 or MDR1genes overexpressed strains（P<0.05）.Compared to parent strains,the up-regulated expression of CAP1 and GRP2were relatively less in CDR1/CDR2 or MDR1genes overexpressed strains,moreover,the down-regulated expression of SOD2 and SOD5 were also relatively less in CDR1/CDR2 or MDR1genes overexpressed strains（P<0.05）.In conclusion,the overexpression of CDR1/CDR2 and MDR1 genes could reduce the oxidative stress response and enhance the adaptability of C.albicans to oxidative stress.更多还原