Objective To determine flavonoids from Selaginella trichoclada Alsto. Methods Flavonoids were isolated and purified by column chromatographies on silica gel column, Sephadex LH-20 column and semipreparative HPLC. Their structures were determined on the basis of their physico-chemical properties and spectroscopic data analysis. Results Five compounds were isolated and elucidated as 4'-O-methylamentoflavone(1), amentoflavone(2), 7, 4', 7, 4'''-tetra-O-methylamentoflavone(3), heveaflavone(4), and 5, 7-dihydrox...