【Objective】 The progenies of Sesamum indicum and wild species were obtained by interspecific hybridization in order to improve charcoal rot disease(Macrophomina phaseolina) resistance of sesame cultivars. 【Method】 No.3 wild sesame(S. indicatum)(P_3), Zhongzhi 14(P_1) and autotetraploid of Zhongzhi 14(P_2) were used as reciprocal cross parents, the F1 plants of interspecific hybrids were obtained using the immature embryo culture technique. First, the authenticity of F1 progenies were confirmed by using phen...