【摘要】 为解决金钱豹(Campanumoea javanica Bl.)丛生芽离体快繁体系在增殖阶段存在可持续继代和可重复性较差的问题,以金钱豹成熟种子萌发后带芽的茎段为外植体,以MS为基本培养基,添加不同质量浓度的6-苄基腺嘌呤(6-benzyladenine,BA)和噻苯隆(Thidiazuron,TDZ)以优化增殖激素配方;分别采用直接生根和间接生根法以获得完整的植株.结果表明:0.5 mg/L BA与低浓度的TDZ(0.01~0.05 mg/L)配合可获得理想的丛生芽,且具有较高的增殖倍数与可持续继代数;最佳植物生长调节剂配方为0.5 mg/L BA+0.02 mg/L TDZ,第5次继代的芽增殖倍数达10.9,可继代次数为10次;直接生根法中诱导的生根率和生根系数分别为28.5%~30.5%和2.5~3.3;间接生根法中不同质量浓度IBA预处理诱导的生根率和生根系数分别为30%~100%和1~5.3,其中300 mg/L IBA溶液浸泡茎段末端15 min,生根率为100%,生根系数为5.3,移栽30 d后成活率为90%.由此表明,所建立的金钱豹丛生芽离体最优快速繁殖体系可持续继代10次,芽增殖倍数保持在10左右;间接生根法诱导生根的效果优于直接生根法,高质量浓度的IBA预处理15 min诱导生根效果最好.更多还原

【Abstract】 In order to solve the problem of poor sustainable subculture and repeatability at propagative stage of Campanumoea javanica Bl,the excised stems with buds from sterile seedlings was used as explants,the MS as base medium and supplemented with different concentration of 6-benzyladenine(BA)and thidiazuron(TDZ) to optimize plant growth regulators(PGRs) prescription.The plantlets were obtained by used the methods of root induction directly or indirectly.The results showed that the combination of 0.5 mg/L BA and low concentration of TDZ(0.01~0.05 mg/L) could induce ideal multiple shoots and had the great proliferative rates and probable subculture number.The best PGRs prescription was 0.5 mg/L BA + 0.02 mg/L TDZ,the proliferative rates at the fifth subculture was 10.9 and the probable subculture number reached to 10.The rooting rate and coefficient was 28.5%~30.5% and2.5~3.3 respectively by using the direct rooting method;30%~100% rooting rate and 1~5.3 rooting coefficient was obtained by used the indirect rooting method,when shoots was pre-treated with different concentration of IBA,the rooting rate and coefficient was reached to 100% and 5.3 respectively after the base of excised shoots were dipped in 300 mg/L IBA solution for 15 min.90% of plantlets survived after30 days’ acclimatization.The results indicated that the optimal in vitro rapid propagation system of C.javanica Bl via multiple shoots could subculture 10 times abidingly and the propliferative rates could kept10;The indirect rooting method was better than the direct one,and that shoots was dipped in high con-centration of IBA solutions for 15 min at the stage of pretreatment could get the best rooting effect.更多还原