【摘要】 为了实现解脂亚罗威亚酵母(Yarrowia lipolytica)直接利用菊粉进行油脂生产,将外切菊粉酶基因INU1与表达质粒p INA1317连接,在解脂亚罗威亚酵母(Y.lipolytica)ACA-DC尿嘧啶缺陷突变菌株中表达。以尿嘧啶缺陷型筛选作为筛选标记获得转化子C37,经过培养菊粉酶酶活达到37.15 U/mL。在2 L发酵罐中,转化子C37以菊粉为底物进行发酵,油脂产量和细胞干质量分别为49%和14 g/L。脂肪酸分析结果显示棕榈酸、硬脂酸和油酸总和占总脂肪酸的92%以上,其中油酸含量高达59%,表明通过菊粉酶基因在解脂亚罗威亚酵母中的表达,实现了以菊粉为底物一步发酵产单细胞油脂。更多还原

【Abstract】 In order to realize oil production directly from inulin by Yarrowia lipolytica,exoinulinase gene INU1 was connected with expression plasmid p INA1317 and expressed in mutant strain Y.lipolytica ACA-DC with uracil deficiency.With uracil deficiency as selection marker,transformant C37 was obtained and the inulase activity was up to 37.15 U/ml by culture.In 2 L fermenter,transformant C37 was fermented with inulin as substrate,the oil yield and cell dry mass were 49% and 14 g/L,respectively.The results of fatty acids analysis showed that the total contents of palmitic acid,stearic acid and oleic acid were more than 92% of total fatty acids,and the oleic acid content was up to 59%,which indicated that the one-step fermentation was realized to produce single cell oil with inulin through the expression of the inulinase gene in Y.lipolytica.更多还原