【摘要】 目的:本研究探索趋化因子CXCL10在免疫介导的肝脏损伤方面的作用及其作用机制。方法:尾静脉高压注射CXCL10表达载体72 h后检测肝脏中CXCL10的表达水平;再尾静脉注射刀豆蛋白(Concanavalin A,ConA)诱导免疫介导的肝脏损伤;检测并比较CXCL10表达组和对照组的谷丙转氨酶(Alanine aminotransferase,ALT)水平、组织损伤水平、IFN-γ水平、TNF-α水平及调节性T细胞比例和数量的差异。结果:CXCL10表达载体注射72 h后,肝脏中CXCL10的表达水平显著升高;CXCL10表达质粒预处理可以有效减轻ConA诱导的肝脏损伤,CXCL10表达组小鼠血清中的ALT水平显著低于对照组,CXCL10表达组小鼠血清中IFN-γ和TNF-α的水平明显低于对照组,CXCL10表达组小鼠肝脏中调节性T细胞比例和数量高于对照组。结论:趋化因子CXCL10表达载体预处理可减少炎性细胞因子并减轻ConA诱导的肝脏损伤,对治疗免疫介导的肝炎具有重要意义。更多还原

【Abstract】 Objective: To explore the effect and mechanism of CXCL10 on immune-mediated liver injury. Methods: The CXCL10 expression vector was injected into mice by hydrodynamic injection. The levels of CXCL10 in the liver were measured 72 hours after injection. Concanavalin A(ConA) was injected into mice to induce immune-mediated liver injury. The levels of alanine a minotransferase(ALT),tissue damage,IFN-γ,TNF-α and percentages and numbers of regulatory T cells were tested and compared between CXCL10 expression group and control group. Results: The levels of CXCL10 in the liver were significantly increased at 72 hours after CXCL10 expression vector was injected. The pretreatment of CXCL10 expression vector markedly reduced ConA-induced ALT levels,IFN-γ levels and TNF-α levels. Additionally,the pretreatment of CXCL10 expression vector increased the percentages and numbers of regulatory T cells in the liver. Conclusion: The pretreatment of CXCL10 expression vector decreases the levels of inflammatory cytokines and attenuates ConA-induced liver injury,which might be beneficial for the treatment for immune-mediated liver injury.更多还原