【摘要】 目的:探讨类风湿性关节炎患者外周血嗜碱性粒细胞的活化情况及其机制。方法:流式检测正常人对照组和RA患者组外周嗜碱性粒细胞活化标记物CD203c的表达及IL-4阳性的比例;用电化学发光法检测血清IgE水平;负选正常人嗜碱性粒细胞,以anti-IgE刺激为阳性对照,置含或不含IgE的微环境中,流式检测CD203c的表达及IL-4阳性的比例。结果:较正常人对照组,RA患者嗜碱性粒细胞高表达CD203c和IL-4(均P<0.05);RA患者血清IgE水平显著高于正常人对照组(P<0.05);RA患者血清和其血清中纯化的IgE可使负选的正常人来源的嗜碱性粒细胞活化,上调CD203c表达及IL-4阳性比例。结论:RA患者嗜碱性粒活化与发病相关,且主要由IgE介导,靶向嗜碱性粒细胞及其活化通路有望为RA的防治提供新的策略。更多还原

【Abstract】 Objective: To investigate the activation of peripheral basophils from patients with rheumatoid arthritis( RA) and its mechanisms. Methods: The activation markers including CD203 c and the proportion of IL-4 positive rate of peripheral basophils from RA patients and healthy controls were detected by flow cytometry. Serum levels of IgE in RA patients and healthy controls were detected by electrochemilu minescence immunoassay. Basophils were negatively isolated and co-cultured with or without purified IgE,anti-IgE as positive control,then,the expression of CD203 c and propotion of IL-4 positive basophils were detected by flow cytometry. Results: The expression of CD203 c and IL-4 positive rate of basophils from RA patients were higher than that of healthy controls( P < 0. 05). Serum levels of IgE in RA were higher than that of healthy controls( P < 0. 05). After co-cultured with isolated IgE from RA patients,basophils negatively isolated from healthy controls were activated,and higher expression of CD203 c and proportion of IL-4( P < 0. 05).Conclusion: Basophil activation is related with development of RA,and its activation is mainly mediated by IgE. Targeting basophil and its activation pathway would be expected to provide new strategies for the treatment of RA.更多还原