【摘要】 以酶法水解冷榨花生粕蛋白质粉制备得到的花生多肽液（<3 000 u）为原料,以氯化亚铁为金属螯合剂,在单因素试验基础上,利用Box-Behnken响应面优化技术对多肽-亚铁螯合条件进行优化分析并获得最优螯合工艺参数,即多肽-亚铁质量比为4.31:1（g/g）,螯合温度为25.4 C,螯合时间28.5 min和螯合pH 7.5,在此优化条件下,多肽-亚铁螯合率为（85.68±1.27）%（n=3）,与模型预测值89.653 1%接近,偏差为4.64%。花生粕蛋白多肽-亚铁螯合物经紫外光谱和红外光谱分析发现,Fe²⁺与多肽中的NH₂⁺以及COO-形成共价配位键并形成稳定的共轭结构,是一种新型有机金属螯合物。更多还原

【Abstract】 Peanut polypeptide liquid（< 3 000 u） that prepared by enzymatic hydrolysis of peanut protein power from cold-pressed peanut meal（CPPM） was raw metarial and the ferrous chloride was metal chelator.The optimal chelate technological parameters was obtained after the the optimized analysis to the polypeptide-ferrous chelator by using Box-Behnken response surface optimization technique based on single factor experiment,ie.,polypeptide-ferrous mass ratio was 4.31:1（g/g）,chelating temperature was 25.4 ℃,chelating time was 28.5 min and chelating pH was 7.5.Under the optimization condition,the polypeptide-ferrous chelation rate was（85.68+1.27）%（n = 3）,which was well agreement with the model predicted value（89.653 1%）,and the deviation between the confirmative result and the predicted result was 4.64%.Through ultraviolet spectroscopy（UV） and infrared spectrometer（FT-IR） analysis,peanut meal albumen polypeptide-ferrous chelate was found,the Fe²⁺ and NH₂⁺ in the polypeptide and COO-form covalence coordinate bond and form stable conjugated structure,which was a new-type organic metal-chelator.更多还原