【摘要】 目的检测ASCL-1基因在肺腺癌组织中的表达情况,分析其与肺神经内分泌标志物表达的相关性,研究ASCL-1成为临床病理诊断肺腺癌伴神经内分泌分化肿瘤标志物的可能性。方法应用免疫组化方法检测283例肺腺癌组织中ASCL-1、神经内分泌标志物(CgA、CD56、Syn)的表达并进行对比分析;同时采用Western blot对其中的63例肺腺癌新鲜组织标本中的ASCL-1蛋白表达进行检测。结果免疫组化显示,ASCL-1在肺腺癌组织中的表达阳性率为16.9%(48/283),CgA、Syn、CD56表达阳性率分别为4.2%(12/283)、22.2%(63/283)、5.3%(15/283)。在肺腺癌组织中ASCL-1的表达与神经内分泌标志物(CgA、Syn、CD56)的表达呈正相关性(0<r<1,P<0.05)。Western blot检测发现9例肺腺癌组织中有ASCL-1蛋白表达,而对应的癌旁正常肺组织中无ASCL-1蛋白表达。结论 ASCL-1基因对肺腺癌伴神经内分泌肿瘤具有相关性,联合检测ASCL-1与CgA、Syn、CD56可提高诊断率。更多还原

【Abstract】 Objective This study is to detect ASCL-1 expression in lung adenocarcinoma( AD),to analyze its expression correlated with traditional neuroendocrine( NE) markers,and to research the possibility of ASCL-1 as clinical pathological markers in AD with NE differentiation. Methods ASCL-1 protein expression by immunohistochemical analysis in 283 cases of AD and compared with expression of traditional NE markers( Chromogranin A、CD56、Synaptophysin). Western blot was applied to detect the expressions of ASCL-1 protein in 63 cases of AD. Results By immunohistochemical,ASCL-1 expression was positive in 48/283( 16. 9%) AD,the positive rate of CgA was 4. 2%( 12/283),the positive rate of Syn was( 63/283),and the positive rate of CD56 was 5. 3%( 15/283).,and positive correlation with NE markers( 0 < r < 1,P < 0. 05). Western blot test in 63 cases of AD and tumor-adjacent normal samples,9 cases of AD express ASCL-1 protein,and tumor-adjacent normal tissue without protein expression. Conclusion ASCL-1 is a new kind of correlative marker which may be applied to clinical pathology diagnosis of AD with NE differentiation,Joint detection with CgA、Syn、CD56may improve the diagnostic rate.更多还原