【摘要】 [目的]应用基因芯片技术研究青少年特发性脊柱侧弯(AIS)来源的骨髓间充质干细胞(BM-MSCs)成脂分化过程中差异表达基因,探究AIS的发病机制。[方法]分离培养AIS患儿及正常对照者BM-MSCs培养至第五代(P5),成脂诱导2周后利用基因芯片技术筛选差异表达基因,并利用RT-PCR技术验证芯片结果。[结果]在AIS及正常来源的BM-MSC成脂分化中共新发现229个基因存在显著差异表达。这些差异表达基因具有细胞粘附、转录调节及信号转导等多种功能。共新发现6条具有显著统计学意义的相关分子通路。[结论]AIS患儿BM-MSCs在成脂分化过程中的基因表达模式与正常对照相比存在明显差异。本研究新发现的差异表达基因及通路可能与AIS的发病相关,对其病因学研究具有重要意义,值得深入研究。更多还原

【Abstract】 [Objectives] To investigate the pathogenesis of adolescent idiopathic scoliosis(AIS) by comparison of gene expression profile of bone marrow mesenchymal stem cells(MSCs) in adipogenic differentiation between AIS patients and normal controls. [Methods] MSCs were isolated and cultivated till the fifth passage(P5) in vitro. Cells were harvested after adipogenic induction. Then the gene expression profile was investigated by microarrays and quantitative RT-PCR. [Results] A total of 229 novel discriminating genes were found significantly different between AIS patients and normal controls. These genes were associated with various functions, including cell adhesion, transcription regulatory activity and signal transduction. Additionally 6 pathways involving adipose regulatory and development were new found. Meanwhile, over-represented GO and KEGG categories being crucial for AIS were identified. [Conclusions] The changes of gene expression in this study could be related to pathogenesis of AIS, so, further in-depth research is needed.更多还原