【摘要】 为探讨中药利湿方剂五苓散对动物体内铜排泄的影响,通过饲喂高铜饲料建立SD大鼠亚慢性铜中毒病理模型,然后用五苓散进行治疗。分别在造模成功和治疗结束时进行心脏采血,火焰原子吸收法测定大鼠血液铜含量,试剂盒法测定血清中AST、LDH和SOD的活性;试验结束时剖杀大鼠,观察内脏器官的病理变化,并取肝脏、肾脏进行组织病理学检查。造模结束时检查发现,与对照组相比,模型组大鼠体质量增长速度减缓,肝脏和肾脏出现明显的病理学变化,血液铜含量极显著升高(P<0.01),说明造模成功。采用五苓散治疗后,与模型组比较,治疗组大鼠肝肾病理学变化明显减轻,血液中铜含量显著降低(P<0.05);SOD活性升高(P<0.05),AST和LDH活性降低(P<0.05);与空白对照组比较,治疗组大鼠上述指标均无显著差异(P>0.05)。上述结果表明,五苓散能有效促进铜中毒大鼠体内铜的排泄,减轻肝、肾组织损伤,可用于临床动物亚慢性铜中毒的治疗。更多还原

【Abstract】 In order to investigate the effects of Wuling San,a Chinese herbal medicine compound powder with promoting diuresis,on the copper excretion in animal,the pathological model of subchronic copper poisoning in SD rats was established by feeding high copper diet,and then Wuling San as a therapeutic drug was given by means of intragastric administration to rats. After the success of building models and at the end of treatment,the blood sample of rats was collected by cardiac puncture for determining copper content with the method of flame atomic absorption spectrometry,and the activities of serum AST,LDH and SOD were detected by reagent kit. At the end of the experiment,all the rats were anatomized to observe the changes of pathologic autopsy of the internal organs,and the histopathologic changes of liver and kidney. Results showed that compared with control group,the rats of the model group were slow in velocity of body weight increasement,the obvious pathological changes of liver and kidney structure were found and the level of blood copper was significantly higher( P < 0. 01),indicating that the model of subchronic copper poisoning in rats was successfully established. After treated with Wuling San,compared with modelgroup,the treatment group had less detectable changes in histopathology,the level of blood copper was significantly lower( P < 0. 05),SOD activity increased( P < 0. 05) and AST and LDH activities declined( P < 0. 05). The above five indexes had no significant differences between the treatment group and the control group( P < 0. 05). The results of this study indicated that Wuling San could effectively promote the excretion of copper in rats,alleviate the damage of liver and kidney,so it could be used to treat clinical subchronic copper poisoning of animals.更多还原