Objective To create a system for prokaryotic expression of a peptidoglycan recognition protein(PGRP)from Staphylococcus aureus and to bioinformatically analyze the expressed product. Methods Based on the sequence of the autolysin gene of S.aureus in GenBank,primers specific for a fragment of the pgrp gene were designed.NCBI Blast was used to analyze the conservation of the gene fragment relative to S.aureus strains in the database.The genomic DNA of S.aureus(ATCC25923)served as a template,and PCR was used t...
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