Objective To use molecular cloning technology to produce recombinant outer membrane protein K(OmpK)of Acinetobacter baumannii in order to provide a basis for further study of the immunological activity of OmpK. Methods PCR was used to amplify the gene coding for OmpK from the ATCC19606 reference strain of A.baumannii,and the gene fragment was cloned into the prokaryotic expression vector pColdI.The recombinant plasmid pColdI/OmpK was transformed into Escherichia coli BL21 cells and its expression was induce...
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