Identification of soluble proteins from adult Trichinella spiralis worms in Western blots of sera from patients with trichinellosis and use of those antigens to diagnose trichinellosis in its early stages
Objective To identify antigens from adult Trichinella spiralis worms in order to promptly and specifically diagnose trichinellosis.Methods Soluble proteins from adult T.spiralis worms were analyzed using SDS-PAGE and Western blots of sera from patients with trichinellosis.Results SDS-PAGE indicated that the soluble proteins had 27 protein bands with a molecular weight(MW)of 95,81.3,73.4,63.6,61.4,55,50.6,47,45.1,43.7,41.9,40.6,39,37.4,35.1,33.3,31,30.4,27.8,26.6,25.4,24,21,19.2,17.9,15.4,and 14.4ku.Western ...
*?**?由于旋毛虫肌幼虫容易通过人工消化法收集,目前国内外对旋毛虫病诊断抗原的研究主要集中在肌幼虫期抗原[1]。国际旋毛虫病委员会(InternationalCommission on Trichinellosis,ICT)推荐用于旋毛虫病血清学诊断的抗原也是肌幼虫排泄分泌(excretory-secretory,ES)抗原[2],?