Objectives To express and purify the toxic protein STM1697 of Salmonella typhimuriumin order to provide a foundation for study of its function.Methods Using the S.typhimurium(ATCC14028)genome as a template,the STM1697 gene was amplified with PCR.After double enzyme digestion,the gene was ligated into the prokaryotic expression vector pGL01.The positive recombinant plasmid was transformed into Escherichia coli BL21(DE3)and its expression was induced with IPTG.The expressed product was purified with a nickel ...
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