目的:研究土茯苓与肿节风不同比例配伍对落新妇苷含量的影响。方法:采用高效液相色谱法测定落新妇苷的含量。色谱柱为Diamonsil C18(2)(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%乙酸(20∶80),流速为1.0 m L·min-1,柱温30℃,检测波长为291 nm,进样量10μL。结果:土茯苓和肿节风配伍共同煎煮,当土茯苓和肿节风的配伍比例为1∶1、3∶1时,提取液中落新妇苷的含量明显增加;当土茯苓和肿节风的配伍比例为1∶3时,提取液中落新妇苷的含量显著降低;其他配伍比例时,含量增加不明显。两药单煎合并也有一定的增溶作用。结论:土茯苓和肿节风配伍提取对落新妇苷的含量有一定的影响,临床使用时以1∶1或3∶1配伍入药为佳,尽量避免1∶3配伍入药。
【英文摘要】
OBJECTIVE To study the effect of different ratios of Smilacis glabrae rhizoma and Sarcandrae herba on contents of astilbin. METHODS The chromatographic method was carried out on a Diamonsil C18(2)(250 mm × 4. 6 mm,5 μm) column at 30℃. The mobile phase was consisted of acetonitrile and 0. 1% acetic acid(20∶ 80). Isocratic elution was performed at the flow rate of1. 0 m L·min-1. The UV detection wavelength was at 291 nm. RESULTS When the compatibility proportions of Smilacis glabrae rhizoma and Sarcandrae her...
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